解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Cucumber seedlings infected with cucumber mosaic virus (CMV) contain a virus-induced RNA-dependent RNA polymerase (RNA replicase) in both soluble and particulate fractions of plant extracts; no such activity is found in uninfected plants. The extensive purification and characterization of the soluble RNA replicase has...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90131-8
更新日期:1981-08-01 00:00:00
abstract::The sequences of 189 residues from the 3' terminus of three RNAs of one strain and two RNAs of another strain of tomato aspermy virus have been determined; there was almost complete sequence homology between the RNAs. A base-paired transfer RNA-like structure is proposed for tomato aspermy virus RNAs which is similar ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90639-5
更新日期:1981-07-15 00:00:00
abstract::The genome of the Bacillus subtilis phage SP01 contains a 12.6-kilobase-pair terminal redundancy. The transcription units in this region, which are utilized in vitro by B. subtilis RNA polymerase holoenzme, have been mapped. In vitro transcripts were separated and characterized by gel electrophoresis. Since there is s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90361-5
更新日期:1981-06-01 00:00:00
abstract::3H-labelled complementary DNA (cDNA) was reverse transcribed from the RNA of three biologically distinct isolates of bean yellow mosaic virus (BYMV-G, Q, and S) by the random primer method of Taylor et al. [Biochim. Biophys. Acta 442, 324-330 (1976)]. Excess virus RNA was hybridized with each of the cDNAs and percenta...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90074-x
更新日期:1981-04-30 00:00:00
abstract::Evidence is presented for the glycosylation of the cauliflower mosaic virus (CaMV) capsid polypeptide. Fluorescent derivatives of concanavalin A (Con A) were shown to bind specifically to the dissociated CaMV capsid polypeptides in polyacrylamide gels. Con A also bound specifically to intact CaMV, so inhibiting the at...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90510-9
更新日期:1981-03-01 00:00:00
abstract::Virions of southern bean mosaic virus (SBMV) show two distinct sensitivity patterns upon heating. Infectivity loss and capsid denaturation occur concurrently if virions are exposed at 50-55 degrees in 0.1 M sodium phosphate buffer, pH 7.5, or 0.1 M glycine-phosphate buffer, pH 9.0. Contrastingly, virions are inactivat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90437-2
更新日期:1981-01-30 00:00:00
abstract::Autoradiography of dodecyl sulfate-impregnated polyacrylamide gels after electrophoresis of the proteins from 32P-labeled cauliflower mosaic virus (CaMV) showed that two of the structural proteins were phosphorylated. These two proteins of 44 and 58 kilodaltons (kd) occur as minor components with some strains of the v...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90294-9
更新日期:1980-11-01 00:00:00
abstract::It has recently been shown that RNA from the bottom component of cowpea mosaic virus is capable of self-replication in cowpea mesophyll protoplasts, providing a tool for the investigation of the contribution of each RNA strand toward viral multiplication. Evidence is presented here, using this system, to show that the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90239-1
更新日期:1980-10-15 00:00:00
abstract::Mixed infections of tobacco protoplasts with Cowpea chlorotic mottle virus (CCMV) and brome mosaic virus (BMV) have been studied. Fluorescent antibody staining showed that capsids of both viruses were made in doubly infected protoplasts but only infectious BMV could be recovered, i.e., BMV dominated in mixed infection...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90050-1
更新日期:1980-09-01 00:00:00
abstract::The DNAs from the granulosis viruses of Plodia interpunctella and Pieris rapae were clearly distinguishable based on restriction endonuclease fragment patterns. By sizing single- and double-digestion products approximate molecular weights of 72 x 10(2) and 75 x 10(6), respectively, were estimated for the viral genomes...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90357-8
更新日期:1980-07-30 00:00:00
abstract::Multiple RNA species (ASV(1-5)) ranging in size from about 61,000 to 185,000 daltons have been detected in association with sunblotch disease of avocado. The predominant species, ASV(5), with a molecular weight of 61,000 accumulates to concentration approaching 5 S RNA. All RNA species are detected in extracts from sy...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90370-0
更新日期:1980-07-15 00:00:00
abstract::Granulin and polyhedrin proteins were purified by preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis from the baculoviruses Autographa californica, Rachiplusia ou, Heliothis zea, Heliothis armigera, Trichoplusia ni, and Spodoptera frugiperda. Antisera were raised against Autographa californica...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90128-2
更新日期:1980-05-01 00:00:00
abstract::Crystals of Cowpea mosaic virus displaying a high degree of order have been grown in two different forms. Hexagonal crystals, space group P6(1)22 or P6(5)22 with a = 450 A and c = 1038 A containing six virus particles per cell, have been grown in sodium citrate buffer at pH 4.9. Each particle has four nearest neighbor...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90513-9
更新日期:1980-02-01 00:00:00
abstract::Soluble RNA-dependent RNA polymerases from healthy and alfalfa mosaic virus (AMV)-infected leaves were purified more than 400-fold from the 100,000g supernatant of leaf homogenates, using ammonium sulfate precipitation, Sephadex G-100 filtration, and chromatography on DEAE Sephadex A25 and P11-phosphocellulose. The DE...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90554-1
更新日期:1980-01-15 00:00:00
abstract::A viroid has been purified from avocado leaves infected by sunblotch disease and designated the avocado sunblotch viroid. It is a covalently closed circular RNA molecule with a molecular weight lower than that of chrysanthemum stunt viroid and citrus exocortis viroid while hybridization analysis with 32P-labeled compl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90045-x
更新日期:1979-11-01 00:00:00
abstract::Ultraviolet absorption spectra and difference spectra were determined for barley stripe mosaic virus (BSMV) virions and protein and for tobacco mosaic virus (TMV) virions and protein in buffer and in sodium dodecyl sulfate (SDS). The absorbance at 260 nm of BSMV virions disrupted in SDS was 92% of that of intact virio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90526-9
更新日期:1979-10-15 00:00:00
abstract::Polyribosomal RNA from tobacco infected with sonchus yellow net virus (SYNV) contained sequences which hybridized to 125I-labeled SYNV RNA and which were complementary to 80 to 100% of the viral RNA genome. The poly(A)-containing RNA from polyribosomes was complementary to over 90% of the viral genome but the polyribo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90375-1
更新日期:1979-08-01 00:00:00
abstract::The major and minor nucleoprotein components in turnip yellow mosaic virus (TYMV) preparations have been fractionated by four cycles of CsCl gradient equilibrium centrifugations. RNAs isolated from the various fractions were translated in a rabbit reticulocyte cell-free system. Three classes of RNAs were studied: geno...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90170-3
更新日期:1979-07-15 00:00:00
abstract::Antisera with Fiji disease virus (FDV) protein-specific and double-stranded (ds) RNA-specific antibodies were prepared by injecting rabbits with highly purified preparations of FDV cores. Electron microscopic examination of negatively stained core preparations disclosed the presence of icosahedral particles, about 54 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90495-1
更新日期:1979-06-01 00:00:00
abstract::The enveloped virions of two strains of a granulosis virus, a synergistic Hawaiian (GVH) and a nonsynergistic Oregonian (GVO) strain which infect the armyworm, Pseudaletia unipuncta, were liberated from their capsules with 0.02 N NaOH and purified by filtration through membrane filters. The envelopes were solubilized ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90439-2
更新日期:1979-04-15 00:00:00
abstract::When DNase-digested calf thymus DNA is used as primer, the RNA-directed DNA polymerase of avian myeloblastosis virus will accept potato spindle tuber viroid (PSTV) as a template for DNA synthesis. The DNA obtained hybridizes specifically to PSTV and low molecular weight RNA isolated from PSTV-infected plants; no hybri...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(78)90180-0
更新日期:1978-09-01 00:00:00
abstract::The 50-70 S RNA complex of a clone of Kirsten murine leukemia virus (Ki-MLV), propagated in NIH 3T3 cells, as well as that of a Moloney (Mo)-MLV, propagated in a mouse thymus-bone marrow cell line, contained an approximately 30 S RNA of unknown biological activity in addition to 38 S genomic RNA. The approximately 30 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(77)90225-2
更新日期:1977-11-01 00:00:00
abstract::The ultrastructure of the mature and immature poxvirus Molluscum contagiosum has been examined by electron microscopy. With freeze-fracturing one fracture plane was observed in the viral envelope. During the conversion of immature into mature virus, particles inside the envelope become partly rearranged into parallel ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(77)90215-x
更新日期:1977-11-01 00:00:00
abstract::Covalently closed circular dimers of phage lambda DNA have been found in Escherichia coli infected with lambda. These dimers can be formed by either the lambda Red or Int systems, by a nonrecombinational replicative mechanism requiring the activity of the lambda O and P genes or by joining of the cohesive ends. Dimers...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(77)90136-2
更新日期:1977-09-01 00:00:00
abstract::Three distinct species of nucleocapsids of equine herpesvirus type-1, designated as either light (L), intermediate (I), or heavy (H) on the basis of their densities in Renografin-76 density gradients (Perdue et al. 1975), were characterized with respect to their amino acid content, DNA content, and role in the maturat...
journal_title:Virology
pub_type: 杂志文章
doi:
更新日期:1976-10-01 00:00:00
abstract::By employing two different buffer solutions for polyacrylamide gel electrophoresis, all genome segments of Fiji disease and maize rough dwarf virus were separated. Fiji disease virus contains ten genome segments with approximate genome molecular weights of 19.26 x 10(6) and 19.85 x 10(6), depending on the buffer emplo...
journal_title:Virology
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::Defective interfering particles of New Jersey serotype VSV were found to contain very little or no virion transcriptase activity. Furthermore no VSV transcriptase activity was observed in cells treated with cycloheximide and infected with DI. Cells infected with DI together with standard infectious virus and cyclohexi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(72)90356-x
更新日期:1972-10-01 00:00:00
abstract::The effects of P2 prophage on lambda-mediated depression of host metabolism, and on lambda macromolecular synthesis have been studied. Experiments employing certain lambdabio deletion mutants and lambda-related phages suggest that the same gene products are involved in inhibition of host metabolism (Hin+ function) and...
journal_title:Virology
pub_type: 杂志文章
doi:
更新日期:1971-11-01 00:00:00
abstract::Induction with ultraviolet light of K12 lysogens carrying defective prophages leads to death of the host cell. In order to determine whether the killing accompanying induction was due to diffusible phage products, or was a result of prophage excision itself, the survival of lysogens carrying DNA defective prophages on...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(68)90012-3
更新日期:1968-01-01 00:00:00
abstract::Aster yellows virus (AYV) was recovered from hemolymph, alimentary canal, salivary glands and ovaries, but not from Malpighian tubules, mycetomes, fat body, testes, or brain of viruliferous leafhoppers, Macrosteles fascifrons (Stål). The presence of virus in the tissues was determined by injecting their extracts into ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(67)90070-0
更新日期:1967-12-01 00:00:00
abstract::A non-plaque-forming vaccine strain of NDV (NDV-B1) interferes with the infection of cultured chick embryo cells by the virulent plaque-forming NDV-L strain. The level of interference increases when the concentration of interfering virus is increased or when the time between infection and superinfection is increased. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(67)90059-1
更新日期:1967-12-01 00:00:00
abstract::The early steps of Rous sarcoma virus-Bryan strain (BS-RSV) infection of genetically resistant and susceptible chick embryo cells were investigated. The methods used were the uptake of virus by cells grown in monolayer, and the ability of infected cells to form foci of transformed cells after plating on resistant and ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(67)90046-3
更新日期:1967-08-01 00:00:00
abstract::Adsorption and one-step growth experiments performed with blue-green algal virus (BGAV) LPP-1 under normal conditions revealed a slow adsorption rate, in comparison with bacteriophage systems, followed by a 7-hour latent period. The burst size was approximately 100 virus particles per cell. The molecular weight of the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(67)90036-0
更新日期:1967-08-01 00:00:00
abstract::A procedure was developed for the purification of the pea enation mosaic virus (PEMV) using differential and rate zonal centrifugation in sucrose density gradients. Infectivity and serological data indicated that the highest virus concentration occurs in infected peas (Pisum sativum) 10-12 days after mechanical inocul...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(66)90060-2
更新日期:1966-03-01 00:00:00
abstract::Maturation of T4 can proceed in the presence of 5-methyltryptophan or puromycin. Puromycin inhibits phage protein synthesis within a few seconds, thus limiting the amount of phage precursor material available for maturation. After the arrest of protein synthesis, maturation continues unabated until the limiting protei...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(66)90050-x
更新日期:1966-03-01 00:00:00